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Preliminary Study
Effect
of hot water extract from Agaricus blazei Murill
on antibody-producing cells in mice
A.
Nakajima-a,
T. Ishida-a,
M. Koga-a,
T. Takeuchi-b,
O. Mazda-c and
M. Takeuchi-a
a- Department
of Biotechnology, Faculty of Engineering, Kyoto Sangyo
University, Motoyama, Kamigamo, Kita-ku, Kyoto 603-8555,
Japan
b- Department of Hygine, Kagoshima
University Faculty of Medicine, Kagoshima, Japan
c- Department
of Microbiology, Kyoto Prefectual University of Medicine, Kyoto,
Japan
Received 17 May 2002; revised 17 May 2002; accepted 19 May 2002. Available
online 17 June 2002.
Abstract
We investigated the immunopotentiating
activities of boiled water-soluble extracts from desiccated Agaricus blazei Murill
(ABM). Effect of ABM extract on antibody production
was investigated by method of hemolytic plaque-forming cells
(PFC) against sheep red blood cells (SRBC) antigen.
ABM extracts significantly (p<0.01)
increased the number of PFC in spleen with intraperitoneal
administration at doses of 25 mg/kg as compared with
control group. The populations of Mac-1- or CD25-positive
cells significantly (p<0.01, p<0.001)
increased, but in CD19-positive cells, there were no differences
in ABM-treated mice as compared with control mice. The expressions
of IL-6 and IL-1β mRNA were augmented by ABM extract in
both peritoneal macrophages and spleen cells. These results suggested
that ABM extract might be an effective stimulator for T cell
and macrophage to IL-1β and IL-6 release, resulting
in augmentation of antibody production against SRBC
antigen.
Author Keywords: Agaricus blazei Murill;
Antibody production; Mice
HIV case study highlights
Case 1:
| Case
1: Male, 53 yr old |
03/05/2003 |
05/14/2003 |
07/09/2003 |
HIV1RNA Ultra (copies/ml) |
1513 |
791 |
186 |
CD4 % |
9 |
12 |
12 |
Body Weight (lbs) |
183 |
206 |
209 |
Case 2:
| Case
2: Male, 52 yr old |
08/27/2003 |
10/01/2003 |
Helper T (CD3) |
6 (L) |
8 (L) |
Helper T (Absolute #) |
157 (L) |
224 (L) |
Ultra Sens Viral Load |
123 |
201 |
Suppressor T (CD8) |
56(H) |
62(H) |
Suppressor T (Abs#) |
1456(H) |
1749(H) |
Case 3:
| Case3:
Male, 48 yr old |
5/21/03 |
10/22/2003 |
HIV-1 QUANTITATION PCR |
46231 |
10986 |
|
